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2 edition of Molecular genetic analysis of the zwf region of cyanobacterial genomes found in the catalog.

Molecular genetic analysis of the zwf region of cyanobacterial genomes

Haydar Karakaya

Molecular genetic analysis of the zwf region of cyanobacterial genomes

by Haydar Karakaya

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Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1995.

StatementHaydar Karakaya.
ID Numbers
Open LibraryOL19085922M

  Molecular population genetics aims to explain genetic variation and molecular evolution from population genetics principles. The field was born 50 years ago with the first measures of genetic variation in allozyme loci, continued with the nucleotide sequencing era, and is currently in the era of population genomics. During this period, molecular population genetics has been revolutionized by. cyanobacterial genomes available and WGS contigs downloadable from NCBI (April ). Hits for each protein were aligned using MAFFT v. 7 (Katoh & Standley ) FFT-NS-i algorithm and the alignments were manually reviewed to remove ambiguous sites, gap regions and short sequences. All alignments were then concatenated into a

Department of Molecular Biology, Saitama University, Shimo-Ohkubo , Saitama , Japan Abstract Chloroplast genome originates from the genome of ancestral cyanobacterial endosymbiont. The comparison of the genomes of cyanobacteria and plants has been made possible by the advance in genome sequencing. Cyanobacteria are a fascinating and versatile group of bacteria of immense biological importance. Thought to be amongst the first organisms to colonize the earth, these bacteria are the photosynthetic ancestors of chloroplasts in eukaryotes, such as plants and algae. In addition, they can fix nitrogen, survive in very hostile environments (e.g. down to degreesC), are symbiotic, have /5(2).

Cyanobacteria are the only known prokaryotes capable of oxygenic photosynthesis, the evolution of which transformed the biology and geochemistry of Earth. The rapid increase in published genomic sequences of cyanobacteria provides the first opportunity to reconstruct events in the evolution of oxygenic photosynthesis on the scale of entire genomes. Here, we demonstrate the overall .   Cyanobacteria are well known for the production of a range of secondary metabolites. Whilst recent genome sequencing projects has led to an increase in the number of publically available cyanobacterial genomes, the secondary metabolite potential of many of these organisms remains elusive. Our study focused on the 11 publically available Subsection V cyanobacterial genomes, .


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Molecular genetic analysis of the zwf region of cyanobacterial genomes by Haydar Karakaya Download PDF EPUB FB2

A 4, bp region around the zwf gene of Anabaena sp. PCC was sequenced. Three genes are located in the region: fop, tal and zwf, which encode fructose-l,6-bisphosphatase (FBPase), transaldolase and glucosephosphate dehydrogenase (G6PDH), respectively.

The fop gene encodes a polypeptide of amino acids. A 4, bp region around the zwf gene of Anabaena sp. PCC was sequenced. Three genes are located in the region: fop, tal and zwf, which encode fructose-l,6-bisphosphatase (FBPase), transaldolase and glucosephosphate dehydrogenase (G6PDH), respectively.

The fop gene encodes a polypeptide of amino : Haydar Karakaya. Molecular genetic analysis of the zwf region of cyanobacterial genomes. By Haydar Karakaya. Molecular genetic analysis of glucosephosphate dehydrogenase (G6PDH) and the other components of the opp cycle have been focused on recently in an effort to understand the function and regulation of the cycle in vegetative cells and in Author: Haydar Karakaya.

The development of these genetic techniques has stimulated interest in cyanobacteria as hosts for the expression of foreign genes. A variety of host-vector systems combined with the ability to clone genes under the control of regulatable promoters, provide the tools required for expression of potentially beneficial genes in by: Genetic Analysis of Cyanobacteria.

Teresa Thiel. I have attempted to assemble a book biology and biochemistry ofthese organisms. Although the It appears that cyanobacterial molecular biologists intervening period had been marked by heated debates have indeed paid attention to the admonition of their over the taxonomy and taxonomic.

Abstract The region of the genome encoding the glucosephosphate dehydrogenase gene zwf was analysed in a unicellular cyanobacterium, Synechococcus sp. PCCand a filamentous, heterocystous cyanobacterium, Anabaena sp. PCC   In this study, gene organization in the zwf regions of the genomes of two cyanobacteria was analysed to establish whether other genes encoding enzymes of dark metabolism are located close to wf and to verify whether the predicted amino acid se- quences of the proteins are consistent with thiore- doxin control of activity.

5 X Denhardt's, % SDS) and washed at 55 in 2 X. Molecular Genetic Analysis of the zwf Region of Cyanobacterial Genomes. University of Warwick, UK. Supervisor Prof. Nick H. MANN MSc Thesis A Floristic and Phytosociological Investigation on the Subalpine and Alpine Vegetations of Çambaşı Highland, Ordu.

Ondokuz Mayis University. Several cyanobacterial strains are also capable of diazotrophic growth. Genome sequencing has provided a large amount of information on the genetic basis of nitrogen metabolism and its control in different cyanobacteria. Comparative genomics, together with functional studies, has led to a significant advance in this field over the past years.

sponsible for genetic transformation was the DNA of the cell—hence that DNA is the genetic material. 4 Chapter 1 Introduction to Molecular Genetics and Genomics Living S cells Living R cells Heat-killed S cells Living R cells plus heat-killed S cells Mouse contracts pneumonia Mouse contracts pneumonia Mouse remains healthy Mouse remains.

Comparative analysis of 15 complete cyanobacterial genome sequences, including “near minimal” genomes of five strains of Prochlorococcus spp., revealed 1, protein families [core cyanobacterial clusters of orthologous groups of proteins (core CyOGs)] encoded in at least 14 of them.

The majority of the core CyOGs are involved in central cellular functions that are shared with other. This volume brings together the expertise and enthusiasm of an international panel of leading cyanobacterial researchers to provide a state-of-the art overview of the field.

Topics covered include: evolution, comparative genomics, gene transfer, molecular ecology and environmental genomics, stress responses, bioactive compounds, circadian clock, structure of the photosynthetic apparatus.

Plant Molecular Biology includes information on plant gene expression and structure, isolation and analysis of chloroplasts, foreign gene expression, molecular plant virology, protoplast isolation and transformation, and molecular biology of cyanobacteria.

The book complements existing molecular biology manuals and will be an essential Format: Paperback. To initiate a comprehensive analysis of cyanobacterial cell division, we first searched cyanobacterial genomes for cell division genes previously identified in E.

coli and B. subtilis and found nine related genes, ftsE, ftsI, ftsQ, ftsW, ftsZ, minC, minD, minE and sulA. As noted above, orthology of cyanobacterial division genes to the last five. Molecular genetic analysis of the zwf region of cyanobacterial genomes.

(Thesis) Karakaya H. Publisher: University of Warwick [] Metadata Source: The British Library Type: Thesis. Abstract. No abstract supplied. Menu. Formats. Abstract. EThOS. About. About Europe PMC; Funders. To investigate the genetic diversity of the genus Arthrospira and to compare it with other cyanobacteria, sequences of nt from the phycocyanin operon were determined for 23 natural, cultivated.

Given that 24 genomes sequences have been completed and many more projects are currently underway, the point has been reached where there is an urgent need to summarise and review the current molecular biology, genomics, and evolution of these important organisms.

Author information: (1)Department of Biological Sciences, University of Warwick, Coventry, UK. The region of the genome encoding the glucosephosphate dehydrogenase gene zwf was analysed in a unicellular cyanobacterium, Synechococcus sp.

PCCand a filamentous, heterocystous cyanobacterium, Anabaena sp. PCC   The First Journal on Genetics, founded in Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic.

Its analysis is widely used for prokaryotic identification in cultivation or in the environment. Cyanobacterial 16S rRNA gene sequences can be obtained by different techniques, with or without cultivation (e.g. PCR followed by clone library, DGGE, Next Generation Sequencing), and they can be compared to sequences of the global databases.

Natural transformation is the process by which bacteria actively take up and maintain extracellular DNA. This naturally occurring process is widely used as a genetic modification method in bacterial species, and is crucial for the efficient genetic modification of organisms in an industrial setting.

Cyanobacteria are oxygenic photosynthetic microbes that are promising platforms for.Analysis of nucleic acids, proteins, and lipids from environmental samples by molecular approaches such as genetic fingerprinting, metagenomics, metaproteomics, metatranscriptomics, and proteogenomics are vital for discovering and characterizing the vast microbial diversity and in understanding their interactions with biotic and abiotic.It would be desirable to have it in libraries or in the book stock of laboratories working in the field of molecular biology and/or photosynthesis or plant phsyiology.' Journal of Plant Physiology, () ` the volume is an excellent summary of information on blue-green algae, their photosynthetic metabolism and its genetic coding.

The.